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Novus Biologicals
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Cyagen Biosciences
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Nissui Pharmaceutical
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Journal: eLife
Article Title: The Fml1-MHF complex suppresses inter-fork strand annealing in fission yeast
doi: 10.7554/elife.49784
Figure Lengend Snippet: Figure 6. Effect of the AAA mutation in Fml1 and D87A mutation in Mhf2 on Fml1 stability. (A) Western blot showing the relative amounts of Fml1+13Myc, Fml1DC-13Myc and Fml1AAA-13Myc in whole cell extracts from strains MCW1221 (fml1+), MCW4406 (fml1+13Myc), MCW6977 (fml1DC-13Myc), and MCW6980 (fml1AAA-13Myc). (B) Western blot showing the relative amounts of Fml1+13Myc/Fml1AAA-13Myc in whole cell lysates from strains MCW1221 (fml1+ mhf2+), MCW9616 (fml1+13Myc mhf2+), MCW9593 (fml1+13Myc mhf2D87A), MCW9594 (fml1AAA-13Myc mhf2+) and MCW9595 (fml1AAA-13Myc mhf2D87A). Fibrillarin serves as a loading control.
Article Snippet: Antibodies, western blotting and Immunoprecipitation Living Colors GFP mouse monoclonal antibody (1:1000) (Clontech Laboratories, CA), PCNA (PC10) mouse monoclonal antibody (1:1000) (Santa Cruz Biotechnology),
Techniques: Mutagenesis, Western Blot, Control